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      느릅나무(Ulmus davidiana var. japonica) 추출물의 물질분리 및 생리활성 탐색 = Isolation and Bioactivity from Ulmus daividiana var. japonica Extract

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      https://www.riss.kr/link?id=T11205186

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      다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

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      Investigations conducted with Ulmus davidiana stem bark have demonstrated it to contain (-)-catchin (Compound 1) and (-)-catechin-7-o-β-D-apiofuranoside (Compound 2), which exhibited antioxidant activity. In the present study, we isolated another compound and identified as (-)-catechin-7-o-β-D-xylopyranoside (Compound 3) by physicochemical and spectrometric methods. The structure of the (-)-catechin-7-o-β-D-xylopyranoside was established primarily by analysis of 1H- and 13C- NMR, HMQC and HMBC.
      Three isolated compounds were analyzed for antioxidant activity using several different model systems, including 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging, hydroxyl radical (OH?) scavenging, SOD-like activity and reducing power. Compounds 1 exhibited strong antioxidant activity on DPPH radicals, with IC50 values of 29.08 μM. The percentage inhibition compounds at 10 μM concentrations on scavenging activity of OH? radicals were 67.82%, 68.22%, 74.71%, respectively. and greater than those 10 μM of BHA(butylated hydroxyanisole), BHT(butylated hydroxytoluene)(57.52%, 56.54%). The reducing power of compound 1 were excellent, and were 1.07 at 1 mM. And we have examined the anti-inflammatory effects. Compound 1 inhibition of LPS-induced NO without appreciable cytotoxicity on the RAW 264.7 cells.
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      Investigations conducted with Ulmus davidiana stem bark have demonstrated it to contain (-)-catchin (Compound 1) and (-)-catechin-7-o-β-D-apiofuranoside (Compound 2), which exhibited antioxidant activity. In the present study, we isolated another com...

      Investigations conducted with Ulmus davidiana stem bark have demonstrated it to contain (-)-catchin (Compound 1) and (-)-catechin-7-o-β-D-apiofuranoside (Compound 2), which exhibited antioxidant activity. In the present study, we isolated another compound and identified as (-)-catechin-7-o-β-D-xylopyranoside (Compound 3) by physicochemical and spectrometric methods. The structure of the (-)-catechin-7-o-β-D-xylopyranoside was established primarily by analysis of 1H- and 13C- NMR, HMQC and HMBC.
      Three isolated compounds were analyzed for antioxidant activity using several different model systems, including 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging, hydroxyl radical (OH?) scavenging, SOD-like activity and reducing power. Compounds 1 exhibited strong antioxidant activity on DPPH radicals, with IC50 values of 29.08 μM. The percentage inhibition compounds at 10 μM concentrations on scavenging activity of OH? radicals were 67.82%, 68.22%, 74.71%, respectively. and greater than those 10 μM of BHA(butylated hydroxyanisole), BHT(butylated hydroxytoluene)(57.52%, 56.54%). The reducing power of compound 1 were excellent, and were 1.07 at 1 mM. And we have examined the anti-inflammatory effects. Compound 1 inhibition of LPS-induced NO without appreciable cytotoxicity on the RAW 264.7 cells.

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      목차 (Table of Contents)

      • Ⅰ. 서론 = 3
      • Ⅱ. 연구사 = 9
      • Ⅲ. 재료 및 방법 = 10
      • 1. 재료 = 10
      • 2. 시약 = 10
      • Ⅰ. 서론 = 3
      • Ⅱ. 연구사 = 9
      • Ⅲ. 재료 및 방법 = 10
      • 1. 재료 = 10
      • 2. 시약 = 10
      • 3. 기기 = 11
      • 4. 추출분획 및 분리 = 11
      • 1) 추출 및 분획 = 11
      • 2) 느릅나무 활성성분 분리 및 분광학적 특성 = 12
      • 5. 항산화 활성 실험 = 14
      • 1) DPPH radical 소거활성 = 14
      • 2) Superoxide dismutase (SOD) 유사활성 = 14
      • 3) Hydroxyl radical 소거활성 = 15
      • 4) Reducing power = 15
      • 6. 항염활성 실험 = 17
      • 1) 항염활성 = 17
      • 2) 세포증식능 검사 = 17
      • Ⅳ. 결과 및 고찰 = 18
      • 1. (-)catechin-7-o-β-D-xylopyranoside의 분광학적특성 = 18
      • 2. Compounds의 항산화 활성 = 30
      • 1) DPPH radical 소석활성 = 30
      • 2) Superoxide dismutase (SOD) 유사활성 = 31
      • 3) Hydroxyl radical 소거활성 = 32
      • 4) Reducing power = 33
      • 3.Compounds의 항염활성 = 35
      • 1) 항염활성 = 35
      • 2) 세포증식능 = 37
      • Ⅴ. 결론 = 38
      • Ⅵ. 참고문헌 = 40
      • Ⅶ. 요약 = 44
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