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      애기장대 전사활성인자 MBF1a의 과발현 형질전환체로부터 비생물학적 및 생물학적 스트레스 내성 연구 = Characterization of abiotic and biotic stress tolerance phenotypes in Arabidopsis overexpressing Multiprotein bridging factor 1a (MBF1a) gene, encoding transcriptional coactivator

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      https://www.riss.kr/link?id=T10868278

      • 저자
      • 발행사항

        광주: 전남대학교 대학원, 2007

      • 학위논문사항
      • 발행연도

        2007

      • 작성언어

        영어

      • 주제어

        MBF1aSAT

      • DDC

        572.8 판사항(22)

      • 발행국(도시)

        광주

      • 기타서명

        Characterization of abiotic and biotic stress tolerance phenotypes in Arabidopsis overexpressing Multiprotein bridging factor 1a (MBF1a) gene, encoding transcriptional coactivator

      • 형태사항

        38p: 삽도; 26cm.

      • 일반주기명

        지도교수:김철수
        참고문헌: p.27-31

      • 소장기관
        • 전남대학교 중앙도서관 소장기관정보
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      다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

      We conducted a yeast genetic screening to identify salt tolerance (SAT) genes in a maize kernel cDNA library. During the screening, we identified a maize clone (SAT41) that seemed to confer elevated salt tolerance in comparison to control cells. SAT41 cDNA encodes a 16-kDa protein which is 82.4 % identical to the Arabidopsis Multiprotein bridging factor 1a (MBF1a) transcriptional coactivator gene. To further examine salinity tolerance in Arabidopsis, we functionally characterized the MBF1a gene and found that dehydration as well as heightened glucose (Glc) induced MBF1a expression. Constitutive expression of MBF1a in Arabidopsis led to elevated salt tolerance in transgenic lines. Interestingly, plants overexpressing MBF1a exhibited insensitivity to Glc and resistance to fungal disease. Our results suggest that MBF1a is involved in stress tolerance as well as in ethylene and Glc signaling in Arabidopsis.
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      We conducted a yeast genetic screening to identify salt tolerance (SAT) genes in a maize kernel cDNA library. During the screening, we identified a maize clone (SAT41) that seemed to confer elevated salt tolerance in comparison to control cells. SAT4...

      We conducted a yeast genetic screening to identify salt tolerance (SAT) genes in a maize kernel cDNA library. During the screening, we identified a maize clone (SAT41) that seemed to confer elevated salt tolerance in comparison to control cells. SAT41 cDNA encodes a 16-kDa protein which is 82.4 % identical to the Arabidopsis Multiprotein bridging factor 1a (MBF1a) transcriptional coactivator gene. To further examine salinity tolerance in Arabidopsis, we functionally characterized the MBF1a gene and found that dehydration as well as heightened glucose (Glc) induced MBF1a expression. Constitutive expression of MBF1a in Arabidopsis led to elevated salt tolerance in transgenic lines. Interestingly, plants overexpressing MBF1a exhibited insensitivity to Glc and resistance to fungal disease. Our results suggest that MBF1a is involved in stress tolerance as well as in ethylene and Glc signaling in Arabidopsis.

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      목차 (Table of Contents)

      • List of Figures ⅰ
      • Abbreviations ⅱ
      • Abstract 1
      • Introduction 2
      • Materials and methods 4
      • List of Figures ⅰ
      • Abbreviations ⅱ
      • Abstract 1
      • Introduction 2
      • Materials and methods 4
      • Results 9
      • Discussion 25
      • References 27
      • Abstract in Korean32
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