Type 2 innate lymphoid cells have a significant impact on chronic atopic dermatitis by regulating skin tissue fibrosis|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Atopic dermatitis is a chronic dermatitis disease, 10% of children and 1-3% of adults are diagnosed. Although it develops due to various complex causes, it is known to be greatly influenced by Th1 and Th2 cells. Depending on the type of acute and chronic atopic dermatitis. The influence of Th1 and Th2 cells are different function and the type of cytokines act dominantly varies. In chronic atopic dermatitis to assisted Th1 cells and type 1 cytokines are usually known as their dominant mechanism. In acute atopic dermatitis, type 2 innate lymphoid cells(ILC2) are known to affect atopic dermatitis, and mechanisms suggest that ILC2 is stimulated by type 2 cytokines such as interleukin-25(IL-25) and Interleukin-33(IL-33), which are secreted by keratinocytes, producing interleukin-4 (IL-4) and interleukin-13 (IL-13). In addition, atopic dermatitis is obligatory accompanied by fibrosis of the dermis, so we would check whether ILC2 affects the fibrosis of the dermis. As far as it's known, fibroblasts have been increasing collagen production by Th2-derived cytokines, resulting in fibrosis. ILC2 is secrete which type 2 cytokines, is also thought to affect skin fibrosis. We used C57BL/6 mice to treat Aspergilus fumigatus for 5 days and progress to 2 days rest for 5 weeks to make chronic atopic dermatitis. and we used transepidermal water loss, skin clinical symptom score , gene expression, histological analysis, collagen assay, total serum IgE, flow cytometrys analysis. Then, to determine whether ILC2 affects atopic dermatitis, chronic atopic dermatitis has been verified using Rag1-/- mice, An experiment was conducted to verify that atopic dermatitis was alleviated in the absence of ILC2 by continuously injecting anti-CD90.2 into Intraperitoneal at intervals of 200µg every two days before treatment of the Af extract into the Rag1-/-mice. As a result, Rag1-/-mice induced chronic atopic dermaltitis models, similar result were found in transepidermal water loss, skin clinical symptom score , gene expression, histological analysis, collagen assay, total serum IgE, flow cytometrys analysis to like wild type C57BL/6 mice.
key words : Chronic atopic dermartitis, helper T cells, type 2 cytokines, type 2 Innate lymphoid cells

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목차 (Table of Contents)

I. Introduction 1
II. Method 2
Murine model of AD 2
Antibody treatment 2
Clinical parameters 2

I. Introduction 1
II. Method 2
Murine model of AD 2
Antibody treatment 2
Clinical parameters 2
Histological examination of the skin 3
Measurement of serum total immunoglobulin E (IgE) 3
Flow cytometry3
Collagen assay 4
Total ribonucleic acid (RNA) preparation and quantitative real-time polymerase chain reaction (RT-PCR) analysis 4
Statistical analysis 5
Ethics statement 5
III. Result 6
AD-like skin inflammation and fibrosis induced by epicutaneous application of Af 6
Type 2 ILCs in skin fibrosis of Af-treated Rag1-/- mice 10
Elimination of type 2 ILCs and skin fibrosis in Af-treated Rag1-/- mice 13
IV. Disscusion 16
V. Reference 18

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Type 2 innate lymphoid cells have a significant impact on chronic atopic dermatitis by regulating skin tissue fibrosis

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