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    A Novel mRNA Platform Produces Robust, Monospecific, Polyfunctional Th1 Responses.

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    https://www.riss.kr/link?id=T17358939

    • 저자
    • 발행사항

      Ann Arbor : ProQuest Dissertations & Theses, 2025

    • 학위수여대학

      University of Pennsylvania Immunology

    • 수여연도

      2025

    • 작성언어

      영어

    • 주제어
    • 발행국

      United States of America

    • 학위

      Ph.D.

    • 페이지수

      80 p.

    • 지도교수/심사위원

      Advisor: Eisenlohr, Lawrence.

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    Helper T cells (TCD4) are lynchpins of adaptive immune responses. Since each TCD4 expresses a single T cell receptor, recognizing specific epitope within major histocompatibility complex class II (MHCII), is often desirable to study a population that responds to the same epitope. We devised a novel method of selective immunization to produce a robust, monospecific TCD4 in mice using a modular mRNA vector. The vector encodes the target epitope attached via flexible linker to MHCII. Immunization with this mRNA selectively elicits TCD4 responses across a range of epitopes and MHCII alleles. These TCD4 show robust, polyfunctional Th1 cytokine release when evaluated in vitro. Additionally, we tested the activity of these TCD4 cells during Salmonella enterica infection, a model of Th1-dependent immune response, and demonstrated their efficacy in vivo in curtailing Salmonella infection.
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    Helper T cells (TCD4) are lynchpins of adaptive immune responses. Since each TCD4 expresses a single T cell receptor, recognizing specific epitope within major histocompatibility complex class II (MHCII), is often desirable to study a population that...

    Helper T cells (TCD4) are lynchpins of adaptive immune responses. Since each TCD4 expresses a single T cell receptor, recognizing specific epitope within major histocompatibility complex class II (MHCII), is often desirable to study a population that responds to the same epitope. We devised a novel method of selective immunization to produce a robust, monospecific TCD4 in mice using a modular mRNA vector. The vector encodes the target epitope attached via flexible linker to MHCII. Immunization with this mRNA selectively elicits TCD4 responses across a range of epitopes and MHCII alleles. These TCD4 show robust, polyfunctional Th1 cytokine release when evaluated in vitro. Additionally, we tested the activity of these TCD4 cells during Salmonella enterica infection, a model of Th1-dependent immune response, and demonstrated their efficacy in vivo in curtailing Salmonella infection.

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