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      고삼(苦參)발효 추출물의 면역활성에 관한 연구 = Studies on Immuno modulating Acitivity of Fermented Sophorae Radix Extract

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      https://www.riss.kr/link?id=A82647600

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      Objectives: This study aims at examining the effect of the fermentative extract of root of Sophorae Radix on the immuno-modulating activity. Methods: Cell viabilities were measured by MTT assay. Effect of SFS on nitric oxide(NO), hydrogen peroxide production from RAW 264.7 cells was accessed by Griess reagent assay. Effect of SFS on productions of inflammatory cytokines such as TNF-α, IL-6 in LPS-induced RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. Results: The results of the experiment are as follows. 1. As a result of carrying out MTT assay to check the cellular toxicity of the fermentative extract of Sophorae Radix. There was not any excessive toxicity to the macrophage when the fermentative extract of root of Sophorae Radix was treated in different concentrations. 2. The fermentative extract of Sophorae Radix increased the generation of hydrogen peroxide in the macrophage and significantly restored the suppression of the generation of the hydrogen peroxide in the macrophage induced by LPS. 3. The fermentative extract of Sophorae Radix reduced the generation of NO in the macrophage and significantly suppressed the increase of the generation of NO in the macrophage induced by LPS. 4. The fermentative extract of Sophorae Radix significantly decreased the amount of TNF-α generated in the macrophage induced by LPS when it was 25 ㎍/mL or higher. Conclusion: These results suggest that SFS has anti-inflammatory moiety related with its inhibition of NO, hydrogen peroxide, TNF-α, IL-6, in macrophage led by LPS.
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      Objectives: This study aims at examining the effect of the fermentative extract of root of Sophorae Radix on the immuno-modulating activity. Methods: Cell viabilities were measured by MTT assay. Effect of SFS on nitric oxide(NO), hydrogen peroxide pro...

      Objectives: This study aims at examining the effect of the fermentative extract of root of Sophorae Radix on the immuno-modulating activity. Methods: Cell viabilities were measured by MTT assay. Effect of SFS on nitric oxide(NO), hydrogen peroxide production from RAW 264.7 cells was accessed by Griess reagent assay. Effect of SFS on productions of inflammatory cytokines such as TNF-α, IL-6 in LPS-induced RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. Results: The results of the experiment are as follows. 1. As a result of carrying out MTT assay to check the cellular toxicity of the fermentative extract of Sophorae Radix. There was not any excessive toxicity to the macrophage when the fermentative extract of root of Sophorae Radix was treated in different concentrations. 2. The fermentative extract of Sophorae Radix increased the generation of hydrogen peroxide in the macrophage and significantly restored the suppression of the generation of the hydrogen peroxide in the macrophage induced by LPS. 3. The fermentative extract of Sophorae Radix reduced the generation of NO in the macrophage and significantly suppressed the increase of the generation of NO in the macrophage induced by LPS. 4. The fermentative extract of Sophorae Radix significantly decreased the amount of TNF-α generated in the macrophage induced by LPS when it was 25 ㎍/mL or higher. Conclusion: These results suggest that SFS has anti-inflammatory moiety related with its inhibition of NO, hydrogen peroxide, TNF-α, IL-6, in macrophage led by LPS.

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