녹용의 지질 성분이 췌도세포의 인슐린 분비에 미치는 영향

임완선 이화여자대학교 대학원 1996 국내석사

卵黃油의 脂質成分에 관한 硏究

김종숙 全南大學校 1989 국내석사

비방사구와 방사구를 수집, 난황만을 분리 채취해 가열 압착하여 얻은 난황유를 chloroform : methanol (2:1, v/v)로 정제한 후 silicic acid column chromatography에 의해 중성 지질, 당지질 및 인지질을 분리하여 그 지질을 Thin layer chromatography와 Gas chromatography로 분획, 정량하여 얻은 결과는 다음과 같다. 1. 난황의 일반성분은 비방사구, 방사구 각각 수분이 49.50%, 47.06%, 조단백질은 16.53%, 16.98%, 조지방은 31.05%, 33.34%, 당질은 1.38%, 1.28%, 회분은 1.54%, 1.34%로 나타났다. 2. 난황유의 화학적 성질은 비방사구, 방사구 각각 산가가 8.95, 9.85, 요오드가는 57.64, 58.15, 비누화가는 240.14, 223.92로 나타났다. 3. 난황유의 지질은 비방사구, 방사구 각각 31.05%, 33.34%였고 중성지질이 96.69% 94.32%, 당지질이 2.20%, 3.04%, 인지질이 1.11%, 2.64%로 나타났다. 4. 중성지질 중에는 비방사구, 방사구 각각 triglyceride가 59.3%, 56.3%로 주성분을 이루었고 그 외에 monoglyceride, diglyceride, free sterol, free fatty acid, esterified sterol과 1가지 미확인 성분이 존재하였다. 5. 당지질 중에는 비방사구, 방사구 각각 digalactosyl diglyceride가 98.3% 97.8%로 주성분을 이루었고 cerebroside 외에 1가지 미확인 성분이 존재하였다. 6. 인지질은 비방사구, 방사구 각각 phosphatidyl choline + phosphathdyl serine이 58.6%, 59.8%로 주성분을 이루었고 그 외에 Lecithin + Sphingomyelin과 2가지 미확인 성분이 존재하였다. 7. 총지질의 주요 지방산 조성은 oleic acid가 43.9%, 43.6%, palmitic acid가 31.6%, 28.2%였고 각 지질의 주요 지방산은 중성지질의 oleic acid 48.5%, 47.6%, palmitic acid 26.6%, 26.7%, 당지질의 palmitic acid 33.7%, 25.7%, 인지질의 oleic acid 23.9%, 29.9%, lauric acid 31.3%, 16.8% palmitic acid 19.4%, 25.7%였다. 8. TLC 상의 중성지질의 triglyceride 주요 지방산 조성은 palmitic acid 75.5%, 72.6%, stearic acid 15.4%, 19.6%였다. Egg Yolk oil was gotten by a roasting and pressure egg yolks abtained from open barn system and cage system, respectively. Lipids in egg yolk oil were extracted with a mixture of chloroform: methanol (2: 1, v / v) and fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography. Components and fatty acid composition of each fraction were determined by thin layer chromatography(TLC) and gas chromatography(GC). The results were summarized as follows: lipid content of egg yolk oil (each cage system (A) and open barn system(B) was 31.05% and 33.34% and the lipid is made up of neutral lipids 96.69 %, 94.32 %, glycolipids 2.20 %, 3.04 % and phospholipids 1.11 %, 2.64 %, respectively. Triglycerides 59.3 %, 56.3 % were the major components among the neutral lipids ; monoglycerides, diglycerides, free sterols, and free fatty acids were the minor components. The major components of glycolipids were digalactosyl diglycerides 98.3 %, 97.8 %, the other components were cerebrosides. The major components of the phospholipids were phosphatidyl choline plus phosphatidyl ethanolamine 58.6 %, 59.8 % the other components were lecithin plus sphingomyelin. The major fatty acids of total lipids were oleic acid 43.9 %, 43.6 % and palmitic acid 31. 6 %, 28.2 %. The major fatty acids of the neutral lipids were oleic acid 48.5 %, 47.6 % and palmitic acid 26.6 %, 26.7 %. The major fatty acids of the glycolipids were palmitic acid 33.7 %, 25.7 %. The major fatty acids of the phospholipids were oleic acid 23.9 %, 29.9 %, lauric acid 31. 3 %, 16.8 %, and palmitic acid 19.4 %, 25.7 %. The major fatty acids of the neutral lipids on TLC were palmitic acid 75.5 %, 72.5 % and stearic acid 15.4 %, 19.6 %.

한국산 검정콩의 지질성분에 관한 연구

이경임 釜山大學校 大學院 1984 국내석사

한국산 검정콩에서 클로로포름-메탄올( 2:1, v/v )용매로 지질을 추출, 정제하여 얻은 총지질을 실리실산 관 크로마토그래피로 중성지질, 당지질 및 인지질을 분획하였으며, 이 분획된 각 지질 성분을 얇은막 크로마토그래피에 의하여 검정콩과 지질성분을 분별 정량 하였고, 각 지질 성분에 대한 지방산 조성을 기체 크로마토그래피로써 분석하였다. 이와 아울러 검정콩의 일반 조성분과 지질의 물리화학적 특성을 살펴본 결과는 다음과 같았다. 1. 검정콩의 일반조성분의 함량은 수분 6.60∼7.85%, 조단백질 36.65∼37.45%, 조지질 18.59∼19.45, 조회분 5.18∼5.36%, 섬유소 4.21∼4.54%, 탄수화물 26.93∼27.19% 였다. 그리고 검정콩 지질의 물리 화학적 특성중 비중은 0.915∼0.916, 굴절율은 1.471∼1.472 였으며 비누화값은 183.4∼185.7, 비비누화물질은 0.69∼0.75%, 요드값은123.8∼126.9, 산값은 0.25∼0.35, 과산화물값은 4.07∼5.99였다. 2 . 검정콩의 총지질의 함량은 22.1∼23.4% 였으며, 이중에서 중성지질은 88.5∼89.2%, 당지질은 2.4∼2.5%, 인지질은 8.4∼9.0 % 였다. 중성지질을 분별 정량한 결과, 트리·글리세리드(80.4%)가 대부분 이었으며, 스테롤 에스테르 및 탄화수소(5.2%), 디·글리세리드(5.0%) 유리지방산(4.8%), 스테롤(4.6%) 등을 함유하고 있었다. 그리고 당지질 성분으로는 모노·갈락토실 디·글리세리드 및 에스테르화된 스테릴 글리코시드(41.9%), 세르브로시드 및 스테릴 글리코시드(16.6%), 디·갈락토실 디·글리세리드(11.8%)등이 분별 정량 되었으며 인지질에서는 포스타티딜 에탄올아민(34.2 %), 포스파티딜 콜린(30.1 %) 포스파티딜 이노지톨(18.9%). 포스파티딜 세린(3.2%) 및 리소포스파티딜 콜린 (2.5 %)이 분별 정량되었다. 3. 분획된 각 지질의 지방산 조성은 중성지질의 경우 리놀레산(53%) 및 올레산(25.6 %)이 주요 구성지방산이었고, 팔미트산(10.8%), 리놀렌산(6.7 %), 스테아르산(3.9 %)등이 함유되어 있었다. 그리고 당지질의 지방산을 리놀레산(50%), 올레산(17.3 %), 팔미트산(17.0 %), 스테아르산(5.0%), 미리스트산(0.56 %)의 순으로 함유되어 있었다. 한편, 인지질의 경우는 리놀레산(63 %)및 팔미트산(21.6%)이 주요 성분이었으며, 올레산(5.9 %), 스테아르산(5.1 %), 리놀렌산(4.2 %)및 미리스트산(0.1 %)으로 구성되어 있었다. A study on the biochemical characteristics of lipids from Korean black soybean was made by the various analytical techniques ( column chromatography, thin layer chromatography and gas chromatography analysis) for the fractionation and determination of lipid classes and fatty acid compositions, The results were summerized as follows : 1 . Total lipids of Korean black soybean consisted of about 88.5 % neutral lipids, 2.5 % glycolipids and 9.0 % phospholipids. 2 . The neutral lipids contained in the total lipids were separated and identified as triglycerides ( 80.4 %), sterol esters, hydrocarbons and others ( 5.2 %), diglycerides ( 5.0 % ), free fatty acids ( 4.8 %), sterols and others ( 4.6 % ). The content of triglycerides as the highest component was calculated as 71.1 % in total lipids. The glycolipids were composed of monogalactosyl diglycerides and esterified steryl glycosides 41.9 %) cerebrosides and steryl glycosides ( 16.6 % ) and digalactosyl diglycerides ( 11.8 %) of which monogalactosyl diglycerides and esteritied steryl glycosides were most abundant. The phospholipids were composed of phosphatidyl ethanolamine ( 34.2 %), phosphatidyl choline ( 30.6 %), phosphatidyl inositol (. 18.9'%I, phosphatidyl serine ( 3.2 % ) and lysophosphatidyl choline ( 2.5 % ). 3 . The major fatty acids in the neutral lipids were linoleic acid ( 53 %) and oleic acid ( 25.6 %), also palmitic acid, linolenic acid and stearic acid were identified, Among the glycolipids, linoleic acid ( 50 % ) oleic acid ( 17.3 %) and palmitic acid ( 17.0%) were predominant fatty acids, And phospholipids had a higher level of linoleic acid ( 63 % ) and palmitic acid ( 21.6 %), a lower level of oleic acid ( 5.9%)

박씨의 脂質成分에 관한 硏究

김선경 全南大學校 1987 국내석사

박씨의 脂質을 chloroform과 methanol (2 ; 1, V/V)로 추출하여 Silicic acid column chromatography법에 의해 中性脂質, 糖脂質, 憐脂質로 分劃하였으며 각 지질층의 성분은 thin-layer chromatography 法에 의해 측정하였는데 그 결과는 다음과 같다. 박씨의 脂質含量은 51.0%이고 그 脂質 중에는 中性脂質(79.15%), 糖脂質(19.32%), 憐脂質(1.53%)로 구성되어 있었다. 中性脂質의 주성분은 triglyceride(87.11%)이었고 diglyceride, monoglyceride, free sterol, free fatty acid를 소량 함유하였다. 糖脂質의 주성분은 monogalactosyl diglyceride (40.81%), steryl glycoside(34.25%), digalactosyl diglyceride(14.81%)이었고, 그 외에 esterified steryl glycoside와 cerebroside를 함유하였다. 憐脂質의 주성분은 phosphatidyl choline (58.21%), phosphatidyl ethanolamine(31.05%)와 그 외에 phosphatidyl inositol(10.74%)를 함유하였다. Lipids of Lagenaria siceraria seed were extracted by the mixture of chloroform-methanol (2: 1 , V/V) and fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography. Components of individual lipid class was determined by thin layer chromatography. The results were follows. Lipid content of Lagenaria siceraria seed was 51.0 % and the lipid consisted of neutral lipids (79.15% ), glycolipids (19.32% ), and phospholipids (1.53). Triglycerides (87.11% ) was the major components among the neutral lipids. Diglycerides, monoglycerides, free sterol and free fatty acids were the minor, components. The major components of glycolipids were monogalactosyl diglycerids (40.81% ), steryl glycosids (34.25% ), and digalactosyl diglycerids (14.81% ) the others were esterified steryl glycosides and cerebrosides. The major components of the phosphol ipids were phosphatidyl cholines (58.21%) , phosphatidyl ethanolamines (31.05% ) and phosphatidyl inositols.(10.74% ).

光州 近郊産 淡水魚의 脂質成分의 分析 : 붕어, 잉어 및 가물치를 중심으로

이병섭 朝鮮大學校 産業大學院 1988 국내석사

These systemic analyses were carried out to investigate approximate compositions, and the isolation and identification of fatty acid in fresh-water fishes (Corassius auratus, Cyprinus carpio and Channa argus) by thin layer chromatography and gas chromatography The results are ; Total lipids were contained 2.9% in Channa argus, 4.4 % of Cyprinus carpio and 5.4 % of Carassius auratus, of which Channa argus had 2.6% free lipid and 0.3 % bound lipid in Chauua argus, and 4.3 % free lipid and 0.1 % bound lipid in Cyprinus carpio, and 5.2 % free lipid and 0.2 % bound lipid in Carassius auratus, respectively. Free forms were mostly consisted of neutral lipids(81.2∼96.3 %) and in bound forms 57.4 %∼73.6 % phospholipids in each sample. TG was the most abundant component, MG. DG. FS. FFA and ES were fractionated and identified in neutral lipids of the free and bound lipid in each sample. The glycolipids contained in free and bound lipids were identified as 7 components, of which MGDG was most abundant in free lipids and DGDG in bound lipids in each sample. The phospholipids in free and bound lipids were identified as 5 components, of which the highest content was PC in free lipid and LT in bound lipid.

인체 피부표면의 지질양과 지질구성성분의 분석에 관한 연구

강호정 이화여자대학교 대학원 1996 국내석사

인체의 피부표면지질은 phospholipids, sphingolipids, choesterols, cholestrolesters, fatty acids, triglycerides, squalene과 wax ester로 구성되며 이들은 체액손실을 막고 경피흡수를 조절하는 외에도 각질 형성세포간의 결합과 탈락 및 표피분화와 성장조절에도 관여한다고 알려져 있다. 본 연구는 정상인과 여드름 환자를 대상으로 피부표면지질의 추출유기용매와 신체부위에 따른 변화를 알아보고 이러한 지질변화가 건조한 피부와 여드름 발생에 미치는 영향을 알아보기 위하여 전자미세저울로 지질의 양을 측정하고 그 구성성분은 high performance thin layer chromatography와 photodensitometer로 측정한 결과는 다음과 같다. 1.Hexane, hexane/methanol(2:3, 용적비) 및 95% ethanol 세가지 용매에 따른 지질추출량은 통계적으로 유의한 차이가 없었다. 다른 용매에 비해 hexane으로 추출시 cholesterol-3-sulfate 및 총 ceramides는 적었고 (p<0.05) triglycerides는 많았다.(p<0.05) 2.이마의 지질량은 다른 부위에 비해 유의하게 많이 추출되었다.(p<0.01) ceramides은 뺨에서 많이 추출되었고 (p<0.05) cholesterol은 다리에서 많았으며 다리나 뺨보다 복부에서 triglycerides는 많았다. Cholesterol esters와 wax esters는 다리에서 뺨과 이마부위보다 적었다(p<0.05). 3.건조한 피부에서의 지질 추출량은 유의한 차이를 보이지 않았다. 건조한 피부보다 매끄러운 피부에서 tryglycerides는 많았지만(p<0.05) 그외 다른 지질구성성분에는 유의한 차이가 없었다. 4.정상인과 여드름 환자에서 지질추출량 및 그 구성성분에 유의한 차이는 없었다. 이상의 결과로 자극성과 홍반발생이 컸던 hexane/methanol 을 사용했을 때 ceramides와 극성지질(polar lipid)인 cholesterol-3-sulfate가 많이 추출되어 ceramides와 극성지질(polar lipid)이 피부장벽기능에 중요한 역할을 할 것이라는 것을 간접적으로 시사하며 겨울철의 피지선 기능감소가 건조한 피부의 원인이 된다고 생각되었다. 여드름 환자는 1차적인 각질층 지질변화에는 영향을 받지않는 것으로 여겨지며, 피부 표면지질의 추출용매에 따른 결과의 해석과 신체부위별 피부표면지질 구성성분의 차이가 피부기초학 연구에 도움이 되리라 여겨진다. The composition and biosynthesis of lipids in human skin have long been of considerable interest. The human skin surface lipids are derived from two principle sources; the sebaceous glands and the epidermis. The composition of skin surface lipids includes phospholipid, sphingolipid, choesterol, cholestrolesters, fatty acids, triglycerides, squalene and wax esters. And it is known that skin surface lipids may play function roles as follows:control of epidermal growth and differentiation, determinination of cohesion and desquamation of corneocyte, formation of lamellar granules and functioning as permeability barrier. Therefore we sat out to find out the differences of skin surface lipids according to the solvents, different anatomic sites and the posible effect of these lipid alteration on xerosis and development of acne. The skin surface lipids was extracted by cup method and analyzed the lipid composition by high perforance thin layer chromatography. The result were as follows: 1.Total amount of lipids extracted with 3 solvents(hexane, hexane/methanol, ethanol) were not significantly different. Hexane/methanol was more powerful extract solvent of epidermal lipids(ceramides & cholesterol ?3-sulfate) than hexane. Hexane/methanol and ethanol was more irritant than hexane. 2.The amount of skin surface lipids on the forehead was higher than that of the other site. The composition of lipids was different according to the sites(total ceramides in cheek and truglycerides in andomen were higher than other sites. Sterol easter and wax esters in leg were lower than other sites) 3.Triglycerides in smooth legs indicates higher proportion than that in xerotic legs. 4.There were no significant differences in amount and composition of extracted lipids between acne patients and normal surbject. In conclusion, these results might ce helpfuul to elucidate the differences of extracted lipid composition depending on the solvents and anatomin sites. Total ceramides and polar lipids may play an important role in the maintenance of the normal barrier function. it is thought that decreased sebaceous function (triglycerides, cholestrol esters and wax esters) would be significantly associated with xerosis of legs. These results suggest that total ceramides primarily have not been implicated in the follicular retention keratinization of acne.

들깨의 脂質成分에 關한 硏究

박기아 同德女子大學校 大學院 1982 국내석사

들기름에 對한 食品學的인 基礎資料를 얻기 위하여 들깨를 脫皮 들깨와 種皮로 나누어 溶媒에 따라 遊離脂質과 結合脂質로 區分抽出하고 silicic acid column ohromatography에 依하여 中性脂質糖脂質 및 燐脂質로 分劃한 뒤 Thin layer 및 Gas Chromatography로 各各의 組成과 脂肪酸 組成을 조사 하였다. 脫皮들깨의 총지질은 54% 이고 種皮는 약23% 였으며, 이 중 脫皮들깨는 99% 이상이 遊離脂質이었고 種皮는 약97%이었다. 遊離脂質에는 中性脂質이 약88%로 제일 많고 糖脂質, 燐脂質의 順이었으며, 結合脂質에는 中性脂質과 극성지질이 거의 동률로 含有되어 있었다. 中性脂質은 ES, TG, FFA, DG, FS, MG로 구성 되었으며 그 중 TG가 약 85%로 가장 많았고, 糖脂質의 구성 성분은 ESG, MGDG, SG, DGDG이었고 燐脂質은 PE, PI, PC, PG, LPC 이었다. 脫皮들깨의 遊離 및 結合脂質을 구성하는 脂肪酸은 linolenic acid가 各各 59.3% 및 50.6%이고 모두 oleic linoleic palmitic, stealic acid의 順이며 불포화지방산이 各各 92% 및 84% 이고, 種皮는 脫皮들깨와 비슷하였으나 linoleic acid가 약간 적고 포화지방산이 많았다. 脫皮들깨 遊離脂質 中 中性脂質, 糖脂質 및 燐脂質의 linolenic acid 含量은 各各 63.8% 50.7% 및 42.1%이며, 結合脂質은 遊離脂質과 같은 경향이나 linolenie acid 含量이 적으며, 種皮는 脫皮들깨와 含量 차이만 있을 뿐 같은 경향이었다. To provide basic informations of perilla oil, lipids of cotyledon and seed coat were fractionated into neutral lipid, glyco lipid and phopho lipid by silicic acid column chromatography. The components and fatty acid composition of each fraction were determined by thin layer chromatography and gas chromatography. The results were summerised as follows; 1. The lipid content in cotyledon part was 54.50%, of which 52.66% was free lipid and 0.12% was bound lipid. Whereas the lipid content in coat was 22.70%, of which 22.69% was free lipid and 0.72% was bound lipid. 2. The content of neutral lipids in the free lipids was about 88% and the rest was composed of glyco lipid and phospho lipid. But the contents of neutral lipid in the bound lipid was almost the same as that of the polar lipids. Contrasting to the free lipid, the phospho lipid content in the bound lipid was larger than that of glycolipid. 3. The neutral lipids in the free and bound lipids consisted of esterified sterol, triglyceride, free fatty acid, diglyceride, free sterol and monoglyceride. The triglyceride (more than 85%) was the main constituent of the neutral lipid. Compositions of neutral lipid of cotyledon were similar to those of coat. 4. Main constituents of glyco lipid both of the free and bound lipids were esterified steryl glycoside, monogalactosyl diglyceride and steroglycoside. And the bound lipid contained esterified steryl glycoside, monogalactosyl diglyceride and steroglycoside in addition to digalactosyl diglyceride. 5. Main constituents of phospho lipid in the free and bound lipids were phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidyl choline. Other cotyledon bound lipid contained a unknown component while the free lipid contained two unknown components and phosphatidyl glycerol. But the coat bound lipid contained lysophosphatidyl choline and the free lipid contained phosphatidyl glycerol. 6. The major fatty acid compositions of perilla lipid were linolenic acid, oleic acid and linoleic acid. The content of those unsaturated fatty acids in perilla lipid as more than 90% (w/w). The content of linolenic acid in perilla lipid was more than 50% (w/w). Linolenic acid content in cotyledon part was higher than that of coat part. 7. The fatty acid compositions of glyco lipid and phospho lipid were similar to those of neutral lipid, whereas unsaturated fatty acid were more abundant in the free lipid than in the bound lipid and also more abundant in cotyledon than in coat. The content of linoienic acid and the other unsaturated fatty acids of cotyledon lipid was slightly higher than that of coat lipid.

호박씨 脂質成分의 系統分析

송근섭 全北大學校 1985 국내석사

호박씨의 脂質을 chlorform-methanol (2 : 1 , v/v)로 抽出한 후 TLC와 GLC로 分離, 定量하여 다음과 같은 結果를 얻었다. 1. 호박씨에는 약 40.12%의 粗脂質이 含有되어 있었으며, 그 중 中性脂質은 93.96%, 糖脂質은 1.92%, 燐謄質은 4.12%이었다. 2. 中性脂質에는 triglyceride (79.50%), free fatty acid (7.74%)가 主成分이였고, 그외 monoglyceride, diglyceride, free sterol, esterified sterol이 含有되어 있었다. 3. 糖脂質에는 cerebroside와 steryl glycoside (52.39%)가 主成分이었고 이 외에 digalactosy diglyceride, monogalactosyl diglyceride, esterified steryl glcoside가 含有되어 있었다. 4. 燐脂質에는 phosphatidyl choline(53.33%)이 主成分이었고, 그외 phosphatidyl inositol과 Phosphatidyl ethanolamine이 含有되어 있었다. 5. 中性脂質의 주요 脂肪酸은 Palmitic acid(18.08%), oleic acid(19.41%) 및 linoleic acid(53.19%)였고, 糖脂質의 주요 脂肪酸은 linoleicacid(22.95%)와 Palmitic acid (48.20%)였으며, 燐脂質의 주요脂防酸은 palmitic acid (24.83%), oleic acid(20.95%) 및 linoleic acid(36.45%)였다. Triglgyceride에는 palmitic acid(19.18%), oleic acid(19.40%) 및 linoleic acid(51.67%)가 主成分이었다. 6. 全脂質의 주요 脂肪酸은 linoleic acid(52.13%), oleic acid(19.45%) 및 palmitic acid (18.65%)였다. 따라서 호박씨 脂質을 linoleic acid가 主成分인 oleic - linoleic oil group으로 分類할 수 있었다. Lipids of pumpkin seed were extracted by the mixture of chloroform-methanol (2 : 1, v/v) and fractionated into lipids, glycolipids, and phospholipids by silicic acid column chromatography. Components and fatty acid composition of individual lipid class were determined by thin layer and gas-liquid chromatographies. 1. Lipid content in pumpkin seed was 40.12%, and total lipid consisted of neutral lipids(93.96%), glycolipids(1.92%) and phospholipids(4.12%). 2. The neutral lipids were identified as triglyceride, free fatty acid, diglyceride, esterified sterol, free sterol and monoglyceride, of which triglyceride and free fatty acid were most abundant and their contents were 79.50% and 7.74%, respectively. 3. The major fatty acids of the neutral lipids were linoleic acid (53.19%), oleic, acid(19.41%) and palmitic acid(18.08%). 4. The glycolipids were identified as cerebroside and steryl glycoside, estrified steryl glycoside, monogalactosyl diglyceride, and digalactosyl, diglyceride, of which the most abundant components were steryl glycoside and cerebroside(53.39%). 5. The major fatty acids of the glycolipids were palmitic acid (48.20%) and linoleic acid (22.95%). 6. The phospolipids were identified as phosphatidyl choline, phosphatidyl inositol and phosphatidyl ethanolamine, of which the most abundant component was phosphatidyl choline(53.33%). 7. The major fatty acids of the phospholipids were linoleic acid (36.45%), palmitic acid (24.83%) and oleic acid(20.95%). 8. The major fatty acids of the triglyceride in pumpkin seed were linoleic acid(51.67%), oleic acid(19.40%) and palmitic acid(19.18%). 9. The major fatty acids of the total lipid weere linoleic acid (52.13%), oleic acid(19.45%) and palmitic acid(18.65%). 10. In view of the fatty acid composition, the pumpkin seed oil was classified as the group of oleic - linoleic oil.

강남콩의 脂質成分에 관한 硏究

김충기 全北大學校 1986 국내석사

강남콩의 脂質을 chloroform-methanol-water(1:2:0.8, v/v)의 溶媒로 抽出하고, 이를 silicic acid column chromatography에 의하여 中性脂質, 糖脂質 및 燐脂質로 分劃한 다음 이들의 脂質組成, 脂肪酸 組成을 thin-layer chromatography, gas-liquid chromatography에 의하여 分離, 定量한 結果 다음과 같은 結論을 얻었다. 1. 강남콩의 總 脂質含量은 乾燥物에 대하여 1.89%이었으며 이중 中性脂質은 48.26%, 糖脂質은 7.48% 燐脂質은 44.26% 이었다. 2. 中性脂質중에는 triglyceride의 含量이 64.64%로 가장 많았고, 그 외에 free sterol, sterol ester, free fatty acid, monoglyceride, 1,3-diglyceride, 1,2-diglyceride와 2가지 미확인된 中性脂質이 含有되어 있었다. 3. 糖脂質중에는 esterified steryl glycoside(38.83%)가 主成分이었고, 그 외에 digalactosyl diglyceride, steryl glycoside, cerebroside, monogalactosyl diglyceride, sulfoquinovosyl diglyceride가 含有되어 있었다. 4. 燐脂質중에는 phosphatidyl choline, phosphatidyl glycerol이 전체 燐脂質의 54.77%을 차지하는 主成分이었고, 그 외에 phosphatidyl inositol, lysophosphatidyl choline, phosphatidyl ethanolamine과 1가지 미확인된 燐脂質이 含油되어 있었다. 5. 全脂質 및 各 脂質 劃分의 주요 脂肪酸은 linolenic acid, linoleic acid, oleic acid, palmitic acid이였다. 특히 linolenic acid는 그 含量이 높아 全脂質의 경우 전체 脂肪酸의 37.08%, 中性脂質의 경우 50.45% 含有되어 있었다 Lipids of kidney bean were extracted by the mixture of chloroform-methanol-water ( 1: 2: 0. 8, v /v ) and fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography. The components and fatty acid composition of these fractions were determined by thin layer chromatography and gas liquid chromatography. 1. Lipid content of kidney bean on a dry weight basis was 1.89 % ,and total lipid was consisted of 48.26% neutral lipids, 7.48 % phospholipids and 44.26 % glycolipids. 2. The neutral lipids were identified as triglyceride, free sterol, sterol ester, free fatty acid, monoglyceride, 1,3-diglyceride and 1,2-digl-yceride, of which triglyceride(64.64%) was the most abundant. 3.The glycolipids were identified as esterified steryl glycoside, digalactosyl diglyceride, steryl glycoside, cerebroside, monogalactosyl diglyceride and sulfoquinovosyl diglyceride, of which esterified steryl glycosde (38. 83 %) was the most abundant. 4.The phospholipids were identified asphosphatidyl choline, phosphatidyl glycerol, phosphatidyl inositol, phosphatidyl ethanolamine and lysophosphatidyl choline, of which phosphatidyl choline and phosphatidyl glycerol were most abundant and their contents were 32. 86% and 21.91 %, respectively. 5.The major fatty acids of kidney bean lipid were linolenic, linoleic, palmitic and oleic acid. Linolenic acid contents were very high to be 37. 08 % in tot al lipid and 50. 45% in neutral lipid.

膽囊 및 總輸膽管 膽石症患者의 膽囊膽汁 脂質成分의 變動에 대한 硏究

김진영 漢陽大學校 1985 국내박사