단와 김동걸 박사 근영 ( 대한본초학회 대한본초학회지 2001년 16권 1호 )

학회자료 대한본초학회 2001 대한본초학회지 Vol.16 No.1

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헌사 ( 대한본초학회 대한본초학회지 2001년 16권 1호 )

이영종 대한본초학회 2001 대한본초학회지 Vol.16 No.1

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우초 안덕균 교수 근영 ( 대한본초학회 대한본초학회지 2001년 16권 1호 )

학회자료 대한본초학회 2001 대한본초학회지 Vol.16 No.2

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委陵菜의 基源에 關한 文獻的 考察

金先熙,安德均 대한본초학회 1986 大韓本草學會誌 Vol.1 No.1

정상사람림프구와 HL-60 cell에서 목향의 세포독성과 백혈병세포 분화효과에 관한 연구

이영준 ( Young Joon Lee ),강수진 ( Su Jin Kang ),구세광 ( Sae Kwang Ku ) 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: This study was focused to investigate the toxicity of Saussurea lappa (SL) extracts in HL-60 cells and human lymphocytes. We also examined the differentiation effect of SL against leukemia cells. Methods: For examining the toxicity of SL, cytokinesis-block micronucleus (CBMN) assay and single cell gel eletrophoresis (SCGE) assay were used in present study. The cell differentiation effect of SL was evaluated by nitroblue tetrazolium (NBT) reduction assay. Results: The inhibition of cell growth in HL-60 cells was observed in a dose-dependant manner after SL treatment for 24 h. According to SCGE assay, HL-60 cells treated with SL increased DNA damage at 10㎍/㎖, while DNA damage was induced by 0.1, 1, 10㎍/㎖concentration of SL in human lymphocytes. Our results indicated that SL have no genotoxic effect in HL-60 cells and human lymphocytes. Additionally, the differentiation effect was induced in 1㎍/㎖SL-treated HL-60 cells. Conclusions: From above results it is suggested that SL could be beneficial for the preparation of the useful agent for treating leukemia.

고삼(苦參)발효 추출물의 면역활성에 관한 연구

김형석 ( Hyung Seok Kim ),한효상 ( Hyo Sang Han ),이영종 ( Young Jong Lee ) 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: This study aims at examining the effect of the fermentative extract of root of Sophorae Radix on the immuno-modulating activity. Methods: Cell viabilities were measured by MTT assay. Effect of SFS on nitric oxide(NO), hydrogen peroxide production from RAW 264.7 cells was accessed by Griess reagent assay. Effect of SFS on productions of inflammatory cytokines such as TNF-α, IL-6 in LPS-induced RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. Results: The results of the experiment are as follows. 1. As a result of carrying out MTT assay to check the cellular toxicity of the fermentative extract of Sophorae Radix. There was not any excessive toxicity to the macrophage when the fermentative extract of root of Sophorae Radix was treated in different concentrations. 2. The fermentative extract of Sophorae Radix increased the generation of hydrogen peroxide in the macrophage and significantly restored the suppression of the generation of the hydrogen peroxide in the macrophage induced by LPS. 3. The fermentative extract of Sophorae Radix reduced the generation of NO in the macrophage and significantly suppressed the increase of the generation of NO in the macrophage induced by LPS. 4. The fermentative extract of Sophorae Radix significantly decreased the amount of TNF-α generated in the macrophage induced by LPS when it was 25 ㎍/mL or higher. Conclusion: These results suggest that SFS has anti-inflammatory moiety related with its inhibition of NO, hydrogen peroxide, TNF-α, IL-6, in macrophage led by LPS.

표고버섯 균사체의 생리활성과 기능성식품 소재 개발

김수민 ( Soo Min Kim ),김은주 ( Eun Ju Kim ) 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: This study was carried out to investigate an antioxidant ability, the change of antioxidant ability, antimicrobial activity and anticancer in functional meat ball and sausage prepared from Lentinus edoes mycelium and texturized soy protein. Methods: This experiments was carried out to investigate antioxidant ability(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180 extracts from Lentinus edoes mycelium. Result: Anticancer ability of Lentinus edoes mycelium showed a 28% survival rate and 63% of inhibition rate of tumor, which showed 1.30±0.4 g of tumor weight. These results revealed an effective Lentinus edoes mycelium resources as anticancer sources. After heating peoducts prepared from Lentinus edoes mycelium, these products doesn`t showed difference between after heating and before heating in measuring of SOD-like activity and DPPH. In DPPH experiment, ethanol extracts showed a high DPPH value as 90.85%, but hot water extracts showed 82.14% in DPPH value. Conclusion: In conclusion, it is very useful resources for preparing functional food on the basis of results from antioxidant(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180.

랫드에서 쌍화탕의 급성독성에 관한 연구

김수정 ( Su Jeong Kim ),이미영 ( Mee Young Lee ),신인식 ( In Sik Shin ),서창섭 ( Chang Seob Seo ),하혜경 ( Hye Kyung Ha ),허정임 ( Jung Im Huh ),신현규 ( Hyeun Kyoo Shin ) 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: This study was conducted to evaluate the acute toxicity and safety of Ssanghwa-tang (Shuanhetang in Chinese, Sou-wa-to in Japanese) in Crl: CD Sprague-Dawley (SD) rat though the current regulatory guideline. Methods: In this study, 10 rats of each sex were randomly assigned to two groups of 5 rats each and were administrated singly by gavage at dose levels of 0 and 2000 mg/kg/day of ssanghwa-tang water extract (SHT). After single administration of SHT, mortalities, clinical signs, body weight changes, gross findings were observed for the 15-day period. Results: Acute toxicity tests revealed that a single oral administration of SHT at dose levels of 2000 mg/kg did not affect clinical signs, body weight, and gross findings, evaluating the safety of SHT. The SHT treatment did not result in any toxicologically significant changes in mortality, clinical signs, body weight changes. Conclusions: These results showed that the single oral administration of SHT did not cause any toxic effect at the dose levels of 2000 mg/kg/day in rats. In conclusion, the median lethal dose (LD50) of SHT was considered to be over 2000 mg/kg/day body for both sexes.

연잎, 연꽃, 연꽃 수술 추출물이 UVB 자외선 조사에 의한 각질형성세포의 보호 및 피부 노화 방지에 미치는 영향

장문석 ( Mun Seog Chang ),고은빛 ( Eun Bit Ko ),이호진 ( Ho Jin Lee ),김주성 ( Ju Sung Kim ),김진수 ( Jin Soo Kim ),지성원 ( Sung Won Jee ),김휴영 ( Hyu Young Kim ),염명훈 ( Myeong Hoon Yeom ),김덕희 ( Duck Hee Kim ),김한곤 ( Han 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts. Methods: Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes. Results: The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at 20 mJ/cm2 UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen. Conclusions: These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.

LPS로 유도한 대식세포의 염증반응에서 우슬의 항염증 효과

김민선 ( Min Sun Kim ),정진수 ( Jin Soo Jeong ),이혜윤 ( Hye Youn Lee ),주영승 ( Young Sung Ju ),배기상 ( Gi Sang Bae ),서상완 ( Sang Wan Seo ),조일주 ( Il Joo Cho ),박성주 ( Sung Joo Park ),송호준 ( Ho Joon Song ) 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: Achyranthes japonica (AJ ) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that AJ could show the anti-inflammatory effects in macrophages. In this experiment, we studied whether AJ could inhibit the inflammatory responses in macrophages. Methods: To measure out the cytotoxicity of AJ, we performed the MTT assay. We evaluated the nitric oxide (NO) production, and cytokine production such as interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. We also investigated the cellular mechanims such as mitogen activated protein kinases (MAPK)s and nuclear factor kappa B (NF-kB). Results: AJ inhibited lipopolysaccharide (LPS)-induced NO production. AJ also inhibited production levels of IL-1b, IL-6 and TNF-a in LPS-stimulated macrophage. Finally, western blot analysis showed that AJ treatment inhibited the activation of p38 but not of extracellular signal-regulated kinase, c-jun NH2-terminal kinase and NF-kB. Conclusions: These results showed that AJ down-regulated the inflammatory response via p38 in macrophages, which suggest that AJ could be a candidate on treating inflammatory diseases.