Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells

Xinyu Wang,Yue Wu,Lin Liu,Hui Bai,Zhiheng Zhang,Mingchao Zhao,Tianwen Ma,Xiaopeng Song,Lina Jia,Liangyu Lv,Yue Yu,Xinyu Xu,Hong Chen,Li Gao 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

Background: Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown. Objectives: This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl. Methods: Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 μg/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated. Results: Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro. Conclusions: This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.

A new plesiomorphic species of webspinner (Embiodea, Clothodidae) from mid-Cretaceous Kachin amber of northern Myanmar

Chen Xinyu,Zhang Huachuan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

A new Mesozoic webspinner (Embiodea) is described and figured based on a moderately well-preserved male in mid-Cretaceous (earliest Cenomanian) amber from northern Myanmar. Perissoclothoda myrrhokaris gen. et sp. nov. is the second fossil representative of the putatively primitive family Clothodidae. It differs from the Cretaceous and more recent Embiodea in several plesiomorphic characters, namely a quadrate mentum, approximately oval submentum, subgenae not meet medially (thus a ventral bridge or gula is lacking), completely symmetrical terminalia, and dimerous cerci. This new find sheds further light on the diversity and morphological disparity of fossil webspinners during the Late Mesozoic. www.zoobank.org/urn:lsid:zoobank.org:pub:11A49D4A-4042-4AA3-858F-919E1A84B87

Efficient degradation of imidacloprid in wastewater by a novel p-n heterogeneous Ag2O/BiVO4/diatomite composite under hydrogen peroxide

Jing Chen,Qifang Ren,Chunshan Xu,Bin Chen,Shaohua Chen,Yi Ding,Zhen Jin,Wanmi Guo,Xinyu Jia 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

In this study, Ag2O/BiVO4/diatomite composite with p-n heterojunction structure was synthesized bysimple hydrothermal method with diatomite as carrier. It was found that under the action of hydrogenperoxide (H2O2), the imidacloprid (IMD) could be effectively degraded by the composite. Systemic evaluationswas conducted on effects of pH value, catalyst dosage, H2O2 concentration, initial IMD concentration,and anion on how effeciently this new photocatalytic composite can degrade IMD. The results showthat the p-n heterojunction formed between the two contact surfaces of Ag2O nanoparticle and BiVO4promotes the charge transfer between the interfaces, inhibits recombination of electrons and holes,and thus significantly improves the catalytic performance. The stability and reusability of Ag2O/BiVO4/diatomite composites were tested with ICP experiment and cyclic experiment. Active substancecapture experiment and ESR reveal the photocatalytic reaction mechanism and confirm that superoxideradical (O2), hydroxyl radical (OH), and h+ can promote the degradation of IMD. The degradation productsand the intermediates of IMD were identified through LC-MS, on a basis of which the possible transformationpathway was proposed. In situ synthesis of p-type Ag2O and n-type BiVO4 heterojunctioncomposites has opened a new direction for photocatalytic degradation of organic pollutants.

First principles study of monolayer Sb2S2Te and a mathematical model of a thin-film thermoelectric generator with maximum power point tracing

Liu Xinyu,Yuan Shaoheng,Gu Bo,Chen Qiongxuan,Pu Xinyu,Zhang Jiangtao 한국물리학회 2022 Current Applied Physics Vol.36 No.-

The result of the structural, electronic and thermoelectric performance of monolayer Sb2S2Te, which is a brannew material, is calculated by using the method of full-potential linearized augmented plane-wave (FP-LAPW), and the exchange-correlation potential selected in this paper is generalized gradient approximation (GGA) scheme. There are no negative frequencies in the calculated phonon spectra, which demonstrates that dynamics of monolayer Sb2S2Te is stable in theory. The average value of lattice thermal conductivity (κl) is smaller than that of other thermoelectric (TE) materials, and monolayer Sb2S2Te has larger figure of merit (ZT) comparing with other well-known materials. According to the TE parameters (Seebeck coefficient S, electrical conductivity σ, electronic thermal conductivity κe and lattice thermal conductivity κl) of monolayer Sb2S2Te, an idealized model of thermoelectric generator (TEG) and a realistic model of maximum power point tracking (MPPT) are built. In addition, this paper studies the influence of different temperature zones, different temperature differences, cross sectional area, height and the number of P–N junction on output power and conversion efficiency of TEG. The results of physical calculation and simulation models show that the conversion efficiency of TEG of monolayer Sb2S2Te is larger than or close to that of other well-known materials, and the tracking accuracy of MPPT proposed in this paper is higher than or close to that of other researches.

Measurement of the beam spot characteristics at the CSNS Back-n white neutron source

Yuan Xinyu,Li Qiang,Fan Ruirui,Chen Yonghao,Qiu Yijia,Yi Han,Liu Chen 한국원자력학회 2026 Nuclear Engineering and Technology Vol.58 No.7

The Back-n white neutron source at the China Spallation Neutron Source (CSNS) plays a significant role in nuclear data measurement, irradiation experiments, and neutron resonance imaging. As one of the key characteristics of the neutron beam, the measurement and detailed analysis of the beam spot are essential. In this study, systematic measurements of multiple beam spots were conducted at different neutron time-of-flight distances in the two experimental stations of Back-n, utilizing a ZnS(6Li) neutron-photon conversion screen coupled with a high-time-resolution iCMOS camera. The study focuses on analyzing the profiles, dimensions, and beam halo characteristics of these commonly used beam modes. These characteristics are fundamental for users conducting various physics experiments, such as nuclear data measurement and neutron imaging, at the back-n white neutron source.

Exosomes as Precise Regulators of the Osteoimmune Microenvironment: Engineering Strategies for Bone Regeneration

He Wang,Ruhua Chen,Xinran Li,Jing Wang,Taiying Chen,Shuqi Sun,Xinjie Qiu,Shaobang Wu,Xinyue Zeng,Zhixin Chen,Xiuyun Ren,Bin Zhao 한국생체재료학회 2026 생체재료학회지 Vol.30 No.-

Bone homeostasis is a dynamically orchestrated process that is intricately regulated by the immune system. The emerging field of osteoimmunology has demonstrated that bone homeostasis and repair are governed by a sophisticated crosstalk between immune and skeletal cells, in which immune signals play a critical role in modulating osteogenesis and osteoclastogenesis. Nevertheless, conventional bone repair strategies frequently overlook immune modulation, instead prioritizing structural support or direct osteoinductive effects. Exosomes—endogenous nanovesicles characterized by low immunogenicity and high bioavailability—have emerged as potent mediators within the immune–bone axis. These vesicles mediate intercellular communication by delivering functional cargo, including miRNAs, proteins, and lipids, across biological barriers, thereby enabling precise regulation of inflammatory responses and immune cell polarization. Importantly, exosomes can reprogram the local immune microenvironment from a pro-inflammatory to a regenerative, anti-inflammatory state, thus promoting enhanced bone healing in complex clinical conditions such as osteoporosis and bone defects. This review systematically examines the molecular mechanisms through which exosomes modulate immune responses in bone biology, highlights their pivotal role in reshaping the osteoimmune microenvironment, and discusses their transformative potential in the development of next-generation, precision-based therapeutic approaches for bone regeneration.

Drilling fluid invasion in shale under chemical potential gradients

Xinyu Guo,Taiheng Zhang,Xiaokai Meng,Ming Tang,Fuyuan Deng,Shimming He,Chunqian Chen 한국자원공학회 2026 Geosystem engineering Vol.29 No.4

Mud shale, characterized by low porosity, low permeability, and high hydration sensitivity, is prone to wellbore instability due to drilling fluid invasion. Previous studies have primarily focused on invasion mechanisms driven by pressure differences and capillary forces, the coupled effects of seepage and chemical-mechanical interactions remain insufficiently explored. To clarify the mechanism of drilling fluid invasion into shale under the coupling of seepage and chemical potential, and its impact on pore pressure, this paper develops a model for drilling fluid intrusion into mud shale, incorporating pressure differences and chemical potential based on seepage mechanics, solved using central and time forward difference methods. The results indicate that the diffusion coefficient and solute concentration decrease with radial distance but increase with temperature, drilling time, and drilling fluid solute concentration. Pore pressure first drops, then rises with radial distance, decreases with fluid solute concentration, and increases with fluid compressibility and wellbore pressure difference. The model provides a method to quantify interactions between drilling fluid and shales and constitutes a useful tool for formulating drilling fluids. It assists in the establishment of proper bottomhole hydraulic and osmotic drilling fluid pressures so as to minimize flow into or out of shales, which could result in wellbore instability problems.

Outcomes of Anti-CD19 CAR-T Treatment of Pediatric B-ALL with Bone Marrow and Extramedullary Relapse

Xinyu Wan,Xiaomin Yang,Fan Yang,Tianyi Wang,Lixia Ding,Lili Song,Yan Miao,Xiang Wang,Yani Ma,Chengjuan Luo,Jingyan Tang,Longjun Gu,Jing Chen,Yanjing Tang,Jun Lu,Benshang Li 대한암학회 2022 Cancer Research and Treatment Vol.54 No.3

PurposeAnti-CD19 chimeric antigen receptor T-cell immunotherapy (19CAR-T) has achieved impressive clinical results in adult and pediatric relapsed/refractory (r/r) B-lineage acute lymphoblastic leukemia (B-ALL). However, the application and effect of CAR-T therapy in B-ALL patients with extramedullary relapse are rarely issued even disqualified in some clinical trials. Here, we examined the efficacy of 19CAR-T in patients with both bone marrow and extramedullary involvement.Materials and MethodsCAR-T cells were generated by transfection of primary human T lymphocytes with a lentiviral vector expressing anti-CD19 single chain antibody fragments (scFvs) with the cytoplasmic domains of 4-1BB and CD3ζ, and used to infuse patients diagnosed as having r/r B-ALL with extramedullary origination. Clinical responses were evaluated by the use of bone marrow aspiration, imaging, and flow cytometry. ResultsEight patients received 19CAR-T infusion and all attained complete remission (CR). Only one patient was bridged to hematopoietic stem cell transplantation (HSCT). Although three patients relapsed after infusion, they received 19/22CAR-T infusion sequentially and attained a second remission. To date, five patients are in continuous CR and all eight patients are still alive. The mean follow-up time was 21.9 months, while the 24-month estimated event-free survival is 51.4%. Conclusion19CAR-T therapy can lead to clinical remission for extramedullary relapsed pediatric B-ALL patients. However, the problem of CD19+ relapses after CAR-T remained to be solved. For patients relapsing after CAR-T, a second CAR-T therapy creates another opportunity for remission for subsequent HSCT.

The associations of job strain and leisure-time physical activity with the risk of hypertension: the population-based Midlife in the United States cohort study

Xinyue Liu,Timothy A. Matthews,Liwei Chen,Jian Li 한국역학회 2022 Epidemiology and Health Vol.44 No.-

OBJECTIVES Job strain is positively associated with incident hypertension, while increasing leisure-time physical activity (LTPA) can reduce incident hypertension. However, the joint associations of job strain and LTPA with incident hypertension among United States workers have yet to be investigated. This study examined the independent and joint associations of job strain and LTPA with incident hypertension. METHODS This prospective cohort study (n=1,160) utilized data from the population-based Midlife in the United States study. The associations of job strain and LTPA at baseline with incident hypertension during follow-up were examined using Cox proportional hazards models. High job strain was derived from a combination of high job demands and low job control, and high LTPA was defined as engagement in moderate or vigorous LTPA at least once per week. RESULTS During 9,218 person-years of follow-up, the hypertension incidence rate was 30.6 (95% confidence interval [CI], 27.3 to 34.3) per 1,000 person-years. High job strain was associated with a higher risk for hypertension than low job strain (adjusted hazard ratio [aHR], 1.29; 95% CI, 1.00 to 1.67). High LTPA was associated with lower hypertension risk than low LTPA (aHR, 0.77; 95% CI, 0.60 to 0.98). Hypertension risk was higher among workers with high job strain and low LTPA than among those with low job strain and high LTPA (aHR, 1.70; 95% CI, 1.18 to 2.43). CONCLUSIONS Job strain and LTPA showed positive and inverse associations, respectively, with incident hypertension. The combination of high job strain and low LTPA was associated with the highest risk for hypertension.

SIMULATING TIRE INFLATION PRESSURE LOSS RATE TEST BY THE IDEAL MATERIAL METHOD

Chen Liang,Xinyu Zhu,Changda Li,Guolin Wang,Liu Ji 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.4

Tire Inflation Pressure Loss Rate (IPLR) test is a widely accepted test method to evaluate the tire pressure retention performance, which helps increase fuel economy and prevent premature tire failures. At present, an IPLR test usually lasts for several months, which greatly reduces the efficiency of relevant research. Several analytical models are available to estimate the IPLR value, however, it mainly considers the gauge and permeability of innerliner, ignoring the effect of other components and detailed tire structure. In order to perform a thorough study of tire pressure loss process, a finite element model was developed with the material input of both mechanical and permeability properties of various rubber compounds and tire geometry input. A creative method, ideal material method, was proposed to describe the transient pressure loss process. Through this solution, a finite element model of Passenger Car Radial tire is established to predict IPLR, with a difference from the experimental result less than 5 %. Based on the model, air diffusion path in the tire is studied to better understand the process, which explains the bubble location in tire immersion tests. The effect of innerliner parameters, including halobutyl content, gauge and ending length of innerliner, on IPLR has been investigated based on simulation models.