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Interleukin-1의 기관지 투여 후 나타나는 폐세척액 내 대식세포의 수적변화에 따른 Xanthine Oxidase의 활성변화
조현국,윤종국,최정목,박원학,이영만,Cho, Hyun-Gug,Yoon, Chong-Guk,Choi, Jeung-Mok,Park, Won-Hark,Lee, Young-Man 한국현미경학회 2001 Applied microscopy Vol.31 No.3
The pulmonary alveolar macrophage is thought to play an important role in the mediation of acute inflammatory lung injury by secretory products including degraded enzymes, cytokines, and reactive oxygen metabolites . This study was conceived to understand the role of alveolar macrophage in oxidative stress induced acute lung injury. To examine the alveolar macrophages and xanthine oxidase (XO) activity in bronchoalveolar lavage fluid (BALF), time-dependent changes of numbers of alveolar macrophages, monocytes and neutrophils in alveolar cavity were counted in association with ultrastructural and cytochemical observations of lung tissue and alveolar cells. The number of monocytes was increased (p<0.001) at 1h after IL-1 treatment compared with that of sham. At 2h after instillation of IL-1, the number of alveolar macrophages was the highest, XO activity in BALF was elevated at 2h after IL-1 instillation and the activity was markedly elevated(p<0.05) at 3h after IL-1 treatment. On the basis of these experimental results, it is suggested that, during early phase of acute lung injury induced by IL-1, alveolar macrophage-derived XO contributes to lung injury earlier than the neutrophilic respiratory burst.
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Effect of Mori Cortex in the Cardiac Injury Induced by Skin Burn
조현국,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2
This study was conducted to investigate an effect of Mori Cortex in the cardiac injury following dermal scald bum in rats. Sprague-Dawley rats were induced scald burn (15% of total body surface area). Heart was removed at 5 h postburn and examined with biochemical assay, ultrastructural observations and stereological analysis. The activity of serum aspartate aminotransferase and creatinine was increased at 5 h postburn compared with them of control. Administration of heat extracts of Mori Cortex after scald bum inhibited the production of KC (neutrophil chemoattractant cytokine) and increased the activity of protein kinase C (PKC) in heart tissue. The activity of myeloperoxidase (MPO) in heart tissue was decreased both at 5 h postburn and in case of Mori Cortex administration after scald burn. Ultrastructurally, many contraction bands and separation of intercalated disk induced by scald bum were decreased by administration of heat extracts of Mori Cortex. In stereological analysis, administration of Mori Cortex after scald burn resulted the volume densities of myofibril and mitochondria were increased compared with them of burn control. These data suggest that Mori Cortex may be a useful stuff to the range of available treatments for cardiac injury induced by skin burn.
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Interleukin-1으로 유도된 흰쥐의 급성 폐손상에서 phospholipase A_2의 활성증가
조현국,문병연 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(B)
In order to investigate the pathogenesis of adult respiratory distress syndrome (ARDS), acute lung injury was induced in rats by insufflation of interleukin-1α (IL-1, 50 ㎍/rat) into the trachea. To identify acute edematous lung injury, five hours after IL-1 administration, lung leak index, lung lavage phospholipid content, and lung lavage protein concentrations were measured. In addition, activities of lung myeloperoxidase (MPO) and phospholipase A_2 (PLA_2), and immunocytochemical changes were examined. IL-1 increased lung leak index, phospholipid content in bronchoalveolar lavage (BAL) fluid, and BAL protein concentrations. Lung PLA_2 activity was significantly increased by IL-1 administration, compared with control. In an immunocytochemical study, PLA_2 antiserum particles were identified in alveolar type Ⅱ epithelial cytoplasm in rats given IL-1 intratracheally. Based on these experimental results, it is suggested that increased PLA_2 activity induced neutrophil-dependent oxidative lung injury and the oxidative lung injury was caused by proinflammatory lipid mediators produced by PLA_2.
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趙顯國 한국중국문화학회 1998 中國學論叢 Vol.7 No.-
本文從現實主義(realism)角度硏究了瞿秋白的<魯迅雜感選集序言>. 瞿秋白在<序言>裏將魯迅文學的現實主義特徵定爲"淸醒的現實主義". 這個槪念在兩個方面上具有其意義與局限. 首先瞿秋白從唯物論o反映論角度解讀魯迅文學和他的思想變化軌迹, 這點置得我們注意. 他以 "正視"和 "熱情"這兩個槪念槪括魯迅現實主義的特徵. 盡管如此, 他過度强調唯物辨證法, 甚至主張사是唯一的世界觀, 從而露出了其局限. 但是他在解讀魯迅文學過程當中, 不但論述了魯迅不斷地關注當時社會現實和人生問題, 以諷刺和幽默對사們進行客觀再現, 進一步還指出了魯迅作品幷不是單純的客觀再現, 而是客觀再現裏삼透着其主觀熱情. 這個觀念是흔耐人尋味的. 瞿秋白認爲這些是淸醒現實主義的基本因素. 령一方面, 瞿秋白在<序言>裏提到了魯迅的思想和文學幷不停留在個人存在和意識的範疇, 而是사還包括魯迅對集體的關心和他個體參與集體的問題, 這點令人注意. 但是瞿秋白對魯迅個人存在意識的複雜性與集體系着如何關係沒有進行更細緻的探討, 這是他的局限.
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Pyrococcus furiosus 유래 고온성 DNA polymerase 유전자의 발현 및 정제
조현국,서동호,정종현,박천석 慶熙大學校 食糧資源開發硏究所 2009 硏究論文集 Vol.28 No.-
현재 Pfu DNA polymerase을 이용한 PCR은 여러 방면에서 많이 사용되는 한 방법으로 자리잡고 있으며 다양한 PCR 방법의 출현으로 다양한 학문에서 널리 쓰이고 있다. 본 연구는 P. furiosus 유래 DNA polymerase를 E. coli에 클로닝 및 발현에 성공하였으며,affinity chromatography를 이용해 손쉽게 Pfu DNA polymerase를 정 제할 수 있었다. 정제된 재조합 Pfu DNA polymerase는 여 러 DNA를 이용해 PCR반응을 수행한 결과,성공적으로 원하는 DNA가 증폭 됨을 확인 되었다. 또한 PCR반응에서 재조합 Pfu DNA polymerase 의 농도가 중요한 factor가 됨을 확인하였다. DNA polymerase gene from Pyrococcus furiosus, an extremely thermophilic archaea, was amplified using PCR. Pyrococcus furiosus DNA polymerase (Pfu) was successfully expressed in E. coli MC1061 and the recombinant enzyme was efficiently purified with Ni-NTA affinity chromatography.Optimal Pfu concentration was studied for the effective PCR reaction.
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