CITES 분류에 의한 사향(麝香)의 기원 동물 분류 및 특성에 대한 고찰

장문석,김도림,김현철,박성규,Chang, Mun Seog,Kim, Do Rim,Kim, Hyun Chul,Park, Seong Kyu 대한한의학방제학회 2016 대한한의학 방제학회지 Vol.24 No.4

Objectives : The purpose of this study was to investigate musk deer in taxonomic status and genus species. We investigated the characteristic of musk deer for medicinal usages. Methods : To identifications of musk deer on the taxonomic status and genus species, the literary investigation were conducted on the Korean, China, and Japan pharmacopoeia and published herbal books, CITES Species Lists too. Results : Musk deer placement in a separate family status is the Moschidae. M. chrysogaster Hodgson and M. sifanicus Przewalski was the same species. So, Alpine musk deer revised M. chrysogaster Hodgson[=M. sifanicus Przewalski]. Geographic distribution of M. moschiferus L. divided Sibirica group and himalaica group. Group himalaica contains three subspecies: Korean musk deer(M. moschiferus parvipes Hol.), Chinese musk deer(M. chrysogaster Hodgson), and Himalayan musk deer(M. leucogaster Hodgson). The genetic divergence between M. moschiferus L. and other species was clearly distinguished from the others. M. berezovskii Flerove was less than the others. However, the divergence among M. chrysogaster Hodgson, M. fuscus Li, and M. leucogaster Hodgson were quite low. Musk deers are mostly distributed around the high-plateau. Moschus were from Nepal, Bhutan, Russia, China. Forest musk deer(M. berezovskii Flerove) farming was conducted in China from 1950s. In the Korean hebal pharmacopoeia, Moschus include l-muscone($C_{16}H_{30}O$ : 238.40) over 2% for quantitative test. Conclusions : There are three species of musk deer, Siberian musk deer(M. moschiferus L.), forest musk deer(M. berezovskii Flerove), and Alpine musk deer(M. chrysogaster Hodgson) for medicinal usages.

산약(山藥)이 정자 운동성 저하에 미치는 영향

장문석,오명숙,이병희,양웅모,김원남,김도림,김향미,박은화,박완수,김윤경,박성규,Chang, Mun-Seog,Oh, Myung-Sook,Lee, Byong-Hee,Yang, Woong-Mo,Kim, Won-Nam,Kim, Do-Rim,Kim, Hyang-Mi,Park, Eun-Hwa,Park, Wan-Su,Kim, Yun-Kyung,Park, Seong-Ky 대한한의학방제학회 2006 대한한의학 방제학회지 Vol.14 No.1

The purpose of this study was to investigate the effect of water extract of Dioscoreae Rhizome on the reproduction activity of Wistar male rat. The group treated with 1.0 g/kg/day oral administrations of water extract of Dioscoreae Rhizoma (SK0l6) during 8 weeks was compared with the normal group. Sperm count, sperm motility, body weight, and testis weight were investigated in two groups. Sperm count. body weight, and testis weight of two groups did not show the significant difference. But sperm motility of the treated group was reduced significantly (the normal group: 56.43 %, SK0l6 group: 53.47 %, p < 0.05). According to the results, SK0l6 have no effects on sperm count, body weight. and testis weight but reduced sperm motility significantly.

쇄양(鎖陽)의 Diphenyl-picryl-hydrazyl (DPPH) 소거 활성 및 HepG2 세포에 대한 항산화 효과

장문석,양웅모,김도림,박은화,박수연,박성규,Chang, Mun-Seog,Yang, Woong-Mo,Kim, Do-Rim,Park, Eun-Hwa,Park, Soo-Yeon,Park, Seong-Kyu 대한한의학방제학회 2007 대한한의학 방제학회지 Vol.15 No.2

The purpose of this study was to investigate the anti-oxidant effect of Cynomorium songaricum. The extract of Cynomorii Herba was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, HepG2 cell viability and $H_2O_2$-induced cytotoxicity by a modified MTT assay. DPPH radical scavenging activity was measured after 30 minutes. The extract was tested by 1. 5, 10, 50, 100 and 500 ${\mu}g/ml$ concentrations. HepG2 cell viability by a modified MTT assay was measured in the concentrations of 10, 50, 100, 250, 500 ug/ml for 24 h. The results showed that the extract scavenged DPPH radical up to 52.2% with 50 ug/ml concentration. The extract did not reduced the cell viability and $H_2O_2-induced$ cytotoxicity (69.4%) was blocked by the extract in the concentrations of 50, 100, 250 and 500 ${\mu}g/ml$. In conclusion, the extract of Cynomorii Herba has potent antioxidant activity.

남성생식세포 Sertoli cell에 미치는 복분자(覆盆子)의 항산화 효과

김영주,장문석,박성규,Kim, Young Joo,Chang, Mun Seog,Park, Seong Kyu 대한한의학방제학회 2018 대한한의학 방제학회지 Vol.26 No.2

Objectives : The purpose of this study was to examine the antioxidant effects of the extract of Rubi Fructus on TM4 Sertoli cells. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity and cell viability assays on Sertoli cells. In addition, hydrogen peroxide-induced oxidative stress on Sertoli cells were examined by MTT assay. The antioxidant enzyme of Cu/Zn SOD, Mn SOD, catalase protein expression on Sertoli cells were also measured. Results : The results showed that the extract scavenged DPPH radical dose-dependent manner. The extract showed no cytotoxicity at concentration of 1, 5, 10, 50, $100{\mu}g/ml$. The hydrogen peroxide-induced cytotoxicity of Sertoli cells was protected to 88.3% by the extract at concentration of $100{\mu}g/ml$. Cu/Zn SOD and Mn SOD protein expression were significantly increased on Sertoli cells, but catalase protein expression was not significantly changed. Conclusions : In conclusion, the extract of Rubi Fructus has antioxidant effects on Sertoli cells and protect male reproductive system against oxidative stress.

Leydig Cell의 항산화에 미치는 벌사상자와 사상자의 비교연구

오지훈 ( Ji Hoon Oh ),김도림 ( Do Rim Kim ),박수연 ( Soo Yeon Park ),장문석 ( Mun Seog Chang ),박성규 ( Seong Kyu Park ) 대한본초학회 2014 대한본초학회지 Vol.29 No.6

Objectives : The purpose of this study was to estimate the antioxidant activity of water extract of Cnidii Fructus (CF) and Torilis Fructus (TF) in Leydig cells. Methods : Free radical scavenging activity of CF and TF against 2,2-diphenyl-1-picrylhydrazyl (DPPH) was determined spectrophotometrically. We investigated the effect of CF and TF in Leydig cells by MTT assay. The protective effects of CF and TF against hydrogen peroxide-induced oxidative stress in Leydig cells. Superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells. Results : The results showed that CF scavenged DPPH radical in a dose-dependent manner by up to 81.2%, TF scavenged DPPH radical in a dose-dependent manner by up to 63.8%. CF showed cell viability as 121.0, 132.7, 126.6% in 5, 10, 100 μg/ml concentrations. TF showed cell viability as 127.5, 111.8% in 5, 100 μg/ml concentraions, respectively. The hydrogen peroxide-induced cytotoxicity of Leydig cells were protected to 86.3% by CF at concentration of 10 μg/ml and protected to 83.5% by TF at concentration of 100 μg/ml. Both CF and TF at all concentrations, SOD activity was not significantly changed. Catalase activity was significantly increased at 10, 100 μg/ml concentrations of CF, respectively. TF`s catalase activity showed no significant difference from that of the control. Conclusions : These results suggest that CF, as an antioxidant, protects Leydig cells in hydrogen peroxide-induced oxidative stress. know that『Kwangjebikeup』played a role in settlement and spreading of foreign knowledge to civilians.

숙지황(熟地黃)이 남성생식세포 GC-1의 항산화에 미치는 영향

장문석 ( Mun Seog Chang ),양웅모 ( Woong Mo Yang ),유태원 ( Tae Weon Yu ),김도림 ( Do Rim Kim ),박은화 ( Eun Hwa Park ),고은빛 ( Eun Bit Ko ),최문정 ( Moon Jung Choi ),김휴영 ( Hyu Young Kim ),오지훈 ( Ji Hoon Oh ),심경준 ( Kyung 대한본초학회 2007 대한본초학회지 Vol.22 No.4

SD계 흰쥐에 대한 마황 추출물의 급성 경구투여 독성 시험 연구

장문석(Mun Seog Chang),심경준(Kyung Jun Shim),오지훈(Ji Hoon Oh),윤지원(Ji Won Yoon),최문정(Moon Jung Choi),김도림(Do Rim Kim),양웅모(Woong Mo Yang),임형호(Hyung Ho Lim),이명종(Myeong Jong Lee),박성규(Seong Kyu Park) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.4

Ephedrae herba(Ma-huang) has been used to treat respiratory conditions for over 5,000 years. The early 1990s, the herbal ephedra and other products containing ephedrine began to be promoted as weight loss aids in United States. The objective of this study was to evaluate the acute toxicity of hebal ephedra in rats. SPF Sprague-Dawley male and female rats were administered orally with herbal ephedra extract of 2,500 mg/kg(low dosage group), and 5,000 mg/kg(high dosage group). We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days. No dead animal and no significant changes of body weights were found during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, hematology, serum biochemistry, and other findings. In conclusion, herbal ephedra extract did not show any toxic effects and oral LD50 value was over 5,000 mg/kg in SD rats.

SD계 흰쥐에 대한 太陰調胃湯의 급성 경구 독성 시험 연구

장문석(Mun Seog Chang),심경준(Kyung Jun Shim),김휴영(Hyu Young Kim),박은화(Eun Hwa Park),고은빛(Eun Bit Ko),최봉재(Bong Jae Choi),양웅모(Woong Mo Yang),임형호(Hyung Ho Lim),이명종(Myeong Jong Lee),박성규(Seong Kyu Park) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.6

The objective of this study was to evaluate the acute toxicity of Taeumjowi-tang in rats. SPF Sprague-Dawley male and female rats were administered orally with Taeumjowi-tang extract of 2,500 mg/kg(low dosage group), and 5,000 mg/kg(high dosage group). We daily examined number of deaths, clinical signs, body weights and gross findings for 14 days. No dead animal and no significant changes of body weights were found during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, hematology, serum biochemistry, and other findings. In conclusion, Taeumjowi-tang extract did not show any toxic effects, and oral LD50 value was over 5,000 mg/kg in SD rats.

연잎 , 연꽃 및 연꽃 수술 추출물의 Tyrosinase 활성억제 및 Melanin 생성억제에 의한 미백 효과

장문석 ( Mun Seog Chang ),김향미 ( Hyang Mi Kim ),양웅모 ( Woong Mo Yang ),김도림 ( Do Rim Kim ),박은화 ( Eun Hwa Park ),고은빛 ( Eun Bit Ko ),최문정 ( Moon Jung Choi ),김휴영 ( Hyu Young Kim ),오지훈 ( Ji Hoon Oh ),심경준 ( Kyung 대한본초학회 2007 대한본초학회지 Vol.22 No.4

연잎, 연꽃, 연꽃 수술 추출물이 UVB 자외선 조사에 의한 각질형성세포의 보호 및 피부 노화 방지에 미치는 영향

장문석 ( Mun Seog Chang ),고은빛 ( Eun Bit Ko ),이호진 ( Ho Jin Lee ),김주성 ( Ju Sung Kim ),김진수 ( Jin Soo Kim ),지성원 ( Sung Won Jee ),김휴영 ( Hyu Young Kim ),염명훈 ( Myeong Hoon Yeom ),김덕희 ( Duck Hee Kim ),김한곤 ( Han 대한본초학회 2011 대한본초학회지 Vol.26 No.2

Objectives: The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts. Methods: Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes. Results: The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at 20 mJ/cm2 UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen. Conclusions: These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.